Journal: Experimental and Therapeutic Medicine

Article Title: Efficacy of vitamin D plus calcium with/without alendronate on bone metabolism in immunologic thrombocytopenic purpura patients with steroid treatment: Nine-month results of a randomized, double-blinded, controlled trial

doi: 10.3892/etm.2019.7694

Figure Lengend Snippet: Impact of ALN on bone metabolism markers. (A-C) Bone formation markers (A) BAP, (B) P1NP and (C) osteocalcin, and (D and E) bone resorption markers (D) TRACP-5b and (E) CTX, were measured at baseline levels and after 1, 3, 6 and 9 months of treatment with CalD or CalD + ALN. *P<0.05, **P<0.01 and ***P<0.001 vs. baseline levels. ALN, alendronate; CalD, caltrate D; BAP, bone-specific alkaline phosphatase; P1NP, type 1 procollagen N-terminal propeptide; TRACP-5b, tartrate resistant acid phosphatase 5b; CTX, C-terminal telopeptides of type 1 collagen.

Article Snippet: Serum bone-specific alkaline phosphatase (cat. no. 48262; BAP; Access Ostase, Beckman Coulter, Inc., Brea, CA, USA), type 1 procollagen N-terminal propeptide (cat. no. E-EL-H0185c; P1NP; Elabscience Biotechnology Co., Ltd, Wuhan, China), tartrate resistant acid phosphatase 5b (cat. no. CSB-E08490h; TRACP-5b; CUSABIO Technology LCC, Wuhan, Hubei, China) and C-terminal telopeptides of type 1 collagen (cat. no. E-EL-H0960c; CTX; Elabscience Biotechnology Co., Ltd, Wuhan, China) were measured with a commercial ELISA.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Carnosol inhibits osteoclastogenesis in vivo and in vitro by blocking the RANKL-induced NF-κB signaling pathway

doi: 10.3892/mmr.2022.12741

Figure Lengend Snippet: Carnosol attenuates OVX-induced bone loss in vivo . (A) H&E staining of femoral sections from the sham, OVX and OVX + carnosol groups 6 weeks following OVX. Scale bar, 500 µm. (B) Micro-CT of femoral sections from the sham, OVX and OVX + carnosol groups 6 weeks following OVX. Scale bar, 1 mm. (C) Quantitative analysis of femoral sections. (D) Serum CTX-1, TRAcp5b and IL-6 levels in the sham, OVX and OVX + carnosol groups 6 weeks following OVX. (E) Serum OCN levels in the sham, OVX and OVX + carnosol groups. **P<0.01, ***P<0.001. OVX, ovariectomy; CTX-1, C-terminal telopeptide; TRAcp5b, tartrate-resistant acid phosphatase type 5b; OCN, osteocalcin; BMD, bone mineral density; BV/TV, bone volume/total volume; Tb.N, trabecular number; BS/TV, bone surface area/total volume.

Article Snippet: IL-6 ELISA Kit (cat. no. BMS603-2, Invitrogen, USA), C-terminal telopeptide (CTX-1) ELISA Kit (cat. no. MBS458686, Mybiosource, USA), tartrate-resistant acid phosphatase type 5b (TRAcp5b) ELISA kit (cat. no. MBS163341, Mybiosource, USA) and osteocalcin (OCN) ELISA Kit (cat. no. MBS725134, Mybiosource, USA) were used for ELISA according to the manufacturer's protocol.

Techniques: In Vivo, Staining, Micro-CT

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1 α /BNIP3 Pathway

doi: 10.1155/2021/6683007

Figure Lengend Snippet: Concentrations of OC, BAP, TRACP-5b, NTX-1, and CTX-1 in each group on the 2nd, 4th, and 8th weeks ( n = 6).

Article Snippet: A rat OC ELISA kit (Cloud-Clone Corp, #SEA471Ra, China), rat BAP ELISA kit (Jiancheng, #A059-1, China), rat TRACP-5b ELISA kit (Cloud-Clone Corp, #SEA902Ra, China), rat NTX-1 ELISA kit (Cloud-Clone Corp. #CEA639Ra, China), rat CTX-1 ELISA kit (Cloud-Clone Corp, #CEA665Ra, China), rat interleukin-2 (IL-2) ELISA kit (CUSABIO, #CSB-E04628r, China), rat tumor necrosis factor- α (TNF- α ) ELISA kit (CUSABIO, #CSB-E11987r, China), rat interleukin-6 (IL-6) ELISA kit (CUSABIO, #CSB-E04640r, China), and rat C-reactive protein (CRP) ELISA kit (CUSABIO, #CSB-E07922r, China) were used to measure the concentrations of bone formation- and bone resorption-specific markers and cytokines in this study.

Techniques: